| 名稱 | CCR5/β-Arrestin/CHO GPCR細(xì)胞 |
| 型號(hào) | CBP71392 |
| 報(bào)價(jià) |  |
| 特點(diǎn) | CCR5/β-Arrestin/CHO |
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電話：4008750250
號(hào)碼：
手機(jī)：18066071954
地址：南京市棲霞區(qū)緯地路9號(hào)
Email： zhangxiangwen@cobioer.com
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藥靶細(xì)胞株 > GPCR細(xì)胞株 > CBP71392CCR5/β-Arrestin/CHO GPCR細(xì)胞
- 詳細(xì)內(nèi)容
| CBP71392 | |
| I. Background | |
| C-C chemokine receptor type 5 (CCR5) is a G protein-coupled receptor and a co-receptor for the entry of human immunodeficiency virus-1 (HIV-1) into cells. CCR5 chemokine receptor is involved in leucocytes chemotaxis to sites of inflammation and plays an important role in the macrophages, T cells, and monocytes recruitment. The chemokine ligands that bind to CCR5 are regulated on activation, normal T Cell expressed and secreted (RANTES) and macrophage inflammatory protein 1 alpha (MIP1α). GenScript’s human CCR5-expressing stable subline is guaranteed to function properly in the calcium flux assay. | |
| II. Introduction | |
| Host Cell: | CHO |
| Stability: | 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12k+10%FBS+5ug/ml puromycin+5ug/ml blasticidin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): | Functional assay for CCR5 receptor |
| Transducer: | Gi/Go family |
| Ⅲ. Description of Host Cell Line | |
| Organism: | Hamster |
| Tissue: | Ovary |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| Ⅳ. Representative Data | |
Figure 1. Dose response of Human CCL3 in CCR5 B-Arrestin CHO-K1 Cell Line (C18). | |
Figure 2. Inhibition of hCCL3-induced Beta-Arrestin Recruitment in CCR5 Beta-Arrestin CHO-K1 Cells (C18). | |
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