名稱 | IL1 Effector Reporter Cell |
型號(hào) | CBP74253 |
報(bào)價(jià) | |
特點(diǎn) | IL1 Effector Reporter Cell |
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電話:4008750250
號(hào)碼:
手機(jī):18066071954
地址:南京市棲霞區(qū)緯地路9號(hào)
Email: zhangxiangwen@cobioer.com
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藥靶細(xì)胞株 > 腫瘤免疫細(xì)胞株 > CBP74253IL1 Effector Reporter Cell
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IL1 Effector Reporter Cell IL1 Effector Reporter Cell IL1 Effector Reporter Cell
I. Background | |
IL-1家族包括11種細(xì)胞因子,7種具有激動(dòng)劑活性的配體(IL-1α,IL-1β,IL-18,IL-33,IL-36α,IL-36β,IL-36γ)和4種具有拮抗活性的成員[IL-1受體拮抗劑(IL-1Rα), IL-36Ra,IL-37,IL-38]。IL-1α,IL-1β,IL-18,IL-33,IL-36α,IL-36β,IL-36γ觸發(fā)MPKK和NF-κB導(dǎo)致炎癥反應(yīng)。而IL-1家族的其他成員具有抗炎作用。IL-1是該家族的一個(gè)標(biāo)志性細(xì)胞因子,它可以共同刺激T輔助細(xì)胞、B細(xì)胞的成熟和增殖、NK細(xì)胞的激活,并與炎癥有關(guān)。IL-1由上皮細(xì)胞、內(nèi)皮細(xì)胞、血小板、成纖維細(xì)胞和免疫細(xì)胞(如巨噬細(xì)胞和中性粒細(xì)胞)產(chǎn)生和分泌,作為先天和適應(yīng)性免疫反應(yīng)的一部分,保護(hù)機(jī)體免受損傷和感染。 | |
II. Description | |
IL1 Effector Reporter Cell 報(bào)告基因藥靶模型很好的模擬了體內(nèi) IL1 的信號(hào)轉(zhuǎn)導(dǎo)過(guò)程, 原理見(jiàn)下圖所示。 Figure 1. IL1 Effector Reporter Cell 細(xì)胞模型原理圖 | |
II. Introduction | |
Expressed gene: | IL1R、IL1RAcP |
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Freeze Medium: | 90% FBS+10% DMSO |
Culture Medium: | RPMI-1640+10%FBS+1μg/ml puromycin+800μg/ml Hygromycin+10μg/ml blasticidin |
Mycoplasma Testing: | Negative |
Storage: | Liquid nitrogen |
Application(s): | Functional(Report Gene) Assay |
III. Representative Data Figure 2. Dose Response of Recombinant Human IL1β in IL1 Effector Reporter Cell (C9). Figure 3. Inhibition of rhIL1β-Induced Reporter Activity by IL1β Neutralization or ILlR Blocking Abs in IL1 Effector Reporter Cell(C9). | |